Therapeutic glycoprotein production in mammalian cells
نویسندگان
چکیده
منابع مشابه
Development of protein-free medium for therapeutic protein production in mammalian cells: recent advances and perspectives
Mammalian cell culture media used for the manufacture of therapeutic protein have advanced systematically from serum-containing into animal-free, protein-free and chemically defined formulations over the past decades. Initially driven by patient safety concerns associated with the use of animal-derived medium components, and later by inconsistent cell culture performance due to variability in p...
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Two-dimensional gel electrophoresis with the 125I-Con A overlay and affinity purification with Con A-agarose revealed the presence of an abundant ubiquitous 100-kDa glycoprotein (GP100) in nucleated mammalin cells. The amount in cultured human and murine cells varies from 3 to 20 X 10(6) molecules per cell making GP100 the most abundant glycoprotein in nucleated cells. Peptide mapping shows tha...
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There is a general consensus that although non-mammalian systems can be effectively employed in the manufacture of many proteins, mammalian systems will have to be chosen for the bulk production of a large number of proteins to be used in therapeutics. The main reason being that only the latter type of system can assure the correct form of post-translational modification that many proteins requ...
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Protein therapeutics, such as antibodies and cytokines, is the largest class of new drug candidates being developed by pharmaceutical companies. Although most of these glycoproteins are produced in mammalian cells, there is concern that their large-scale production could be affected by an inadequate supply of bovine serum. There is also the risk of viral infection spreading through the use of c...
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Single chain Fv (scFv) antibody fragments fused to the human immunoglobulin G (IgG) Fc moiety obtain IgG-like properties but nevertheless they are still encoded by a single gene. Since transient production of scFv-Fc proteins in mammalian can easily achieve milligram amounts, this antibody format is particularly suitable for many research applications. Here, we describe two protocols for the tr...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2017
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2017.04.028